Automated Multiple Development (AMD2)

AMD 2

AMD 2

Automated Multiple Development (AMD2)

Only the AMD procedure can be successfully employed for reproducible gradient elution with silica gel as the stationary phase. In column liquid chromatography, gradient elution is common on reversed phases only because a silica gel column would call for a time consuming reconditioning or be irreversibly degraded, which is not acceptable in a technique depending on multiple use of the stationary phase. In thin-layer chromatography this is not relevant.

The principle of the CAMAG AMD procedure

The principle of the CAMAG AMD procedure

  • The HPTLC plate is developed repeatedly in the same direction.
  • Each successive run extends over a longer solvent migration distance than the one before.
  • Between runs, the solvent is completely removed from the developing chamber and the layer is dried under vacuum.
  • Each successive run uses a solvent of lower elution strength than that of the one used before. In this way, a stepwise elution gradient is formed.
  • The combination of focusing effect and gradient elution results in extremely narrow bands. Their typical peak width is about 1 mm. This means that, with the available separation distance of 80 mm, up to 40 components can be completely resolved, i.e. with base line separation.

022.8860: CAMAG AMD2-System comprising chromatogram developing module, AMD 2 EquiLink and accessories; operating voltage 115V nominal (90-130V) or 230V nominal (180-260V) internal jumper selectable, 50/60 Hz, Accessories include all electrical and pneumatic connecting elements, supply of sealing materials, HPTLC plate positioning device, and small ancillaries Dimensions: 430x500x550 mm (WxDxH), Weight: 31 kg

027.6300: winCATS License including 1 year Internet Update Service

022.8880: Oil-sealed rotary vane pump RV3 (Edwards) including gas ballast oil return,oil vapor filter, flexible vacuum hose (1m), and connector to the AMD

 

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